29th Annual CBB Conference
October 22, 2022
1. Poly (ADP-ribose) Polymerase 1: DNA complexes are dependent on DNA sequence and secondary structure
Fletcher Bain, Jade Miller, and Maria Spies*
Department of Biochemistry and Molecular Biology, Carver College of Medicine, University of Iowa, Iowa City, IA
2. NOVEL ANALOGUES OF THE NATURAL PRODUCT FRAXINELLONE PROTECT AGAINST GLUTAMATE- INDUCED TOXICITY IN PC12 CELLS
Anna E. Bartman1, Mersad Raeisi2, Zetandro Banarjee2, Clarence D. Peiris2, Anayah Ferris2, Emmanuel Bonsu, David B. Martin2*, and Jonathan A. Doorn1*
1Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy University of Iowa, Iowa City, IA
2Department of Chemistry, College of Liberal Arts and Sciences, University of Iowa, Iowa City, IA
3. EVALUATING IMPACT OF STIRRING ON THE STABILITY OF PROTEIN PHARMACEUTICALS WITH BOVINE SERUM ALBUMIN (BSA) AS A MODEL PROTEIN
Apurva C. Dusane and M. Reza Nejadnik*
Department of Pharmaceutics and Experimental Therapeutics, College of Pharmacy, University of Iowa, Iowa City, IA
4. A GLOBAL SIDE-CHAIN OPTIMIZATION METHOD TO DETERMINE PROTONATION STATES OF TITRATABLE AMINO ACIDS
Rose A. Gogal, and Michael J. Schnieders*
Department of Biomedical Engineering, College of Engineering, University of Iowa, Iowa City, IA
5. ALLOSTERIC TUNING OF CASPASE-7: ESTABLISHING THE NEXUS OF STRUCTURE AND CATALYTIC POWER
Kathryn F. Hobbs1, Jonah Propp2, Nicholas R. Vance2, Andrew Kalenkiewicz1, Katie R. Witkin2, and M. Ashley Spies1,2*
1Biochemistry and Molecular Biology, Carver College of Medicine, University of Iowa, Iowa City, IA
2Department of Pharmaceutics and Experimental Therapeutics, College of Pharmacy, University of Iowa, Iowa City, IA
6. Dual Targeting of mTOR and YAP/TAZ will synergistically inhibit PI3K-driven sarcomagenesis
Keith Garcia1†,Ali Khan1†, Nicole Merritt1, Colleen Fullenkamp1, Nicholas P. Scalora1, Jordan Kohlmeyer1, Mariah Leidinger1, Michael Chimenti2, and Munir Tanas1
1Department of Pathology, Carver College of Medicine, University of Iowa, Iowa City, IA,
2Iowa Institute of Human Genetics, Carver College of Medicine, University of Iowa, Iowa City, IA.
† these authors contributed equally
7. DEVELOPMENT OF 3D PRINTED GENE-ACTIVATED CALCIUM PHOSPHATE CEMENT SCAFFOLDS FOR APPLICATION IN BONE REGENERATION
Noah Z. Laird1, Esraa Mohamed1, ^, Pornpoj Phruttiwanichakun1, ^, Timothy M. Acri1, Jaidev L. Chakka1, Douglas Fredericks2, John M. Femino2, and Aliasger K. Salem1, *
1Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, University of Iowa, Iowa City, IA
2Department of Orthopedics and Rehabilitation, Carver College of Medicine, University of Iowa, Iowa City, IA
^These authors contributed equally.
8. Double-Stranded mRNA Lipid Nanoparticle Vaccines Display Superior Thermostability Compared to Current Single-Stranded mRNA Formulations
Kristopher E. Lukas and Kevin G. Rice*
Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, University of Iowa, Iowa City, IA
9. APPLICATION OF LYOPHILIZED GENE-DELIVERY FORMULATIONS TO DENTAL IMPLANT SURFACES: NON-CARIOGENIC LYOPROTECTANT PRESERVES TRANSFECTION ACTIVITY OF POLYPLEXES LONG- TERM
Walla I. Malkawi1, Noah Z Laird1, Satheesh Elangovan2, and Aliasger K. Salem1*
1Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, University of Iowa, Iowa City, IA
2Department of Periodontics, College of Dentistry and Dental Clinics, University of Iowa, Iowa City, IA
10. CATIONIC CaMKIIN-LOADED LIPOSOMES AIMED TO REDUCE CHLORINE-INDUCED AIRWAY OXIDATIVE STRESS
Esraa Mohamed1, Andrea Adamcakova-Dodd2, Isabella Grumbach3, Peter S. Thorne2,4, and Aliasger K. Salem1,5*.
1Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, University of Iowa, Iowa City, IA
2Department of Occupational and Environmental Health, College of Public Health, University of Iowa, Iowa City, IA
3Abboud Cardiovascular Research Center, Department of Internal Medicine, Division of Cardiovascular Medicine, Carver College of Medicine, University of Iowa, Iowa City, IA
4Human Toxicology Program, Graduate College, University of Iowa, Iowa City, IA
5Holden Comprehensive Cancer Center, University of Iowa Hospitals and Clinics, University of Iowa, Iowa City, IA
11. Design and Synthesis of Small Molecules that Target UNC119 for the Treatment of Diabetes Mellitus
Autumn E. Moore1, Aschleigh Graham1, Julien A. Sebag2, Nikolai Artemyev2, and Robert J. Kerns1*
1Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, University of Iowa, Iowa City, IA
2Department of Molecular Physiology and Biophysics, Carver College of Medicine, University of Iowa, Iowa City, IA
12. USE OF A SMALL MOLECULE (LM11A-31) INHIBITOR OF p75NT RECEPTOR TO ASSESS THE MECHANISM OF PBD, A NEUROTROPHIC NATURAL PRODUCT
Bishnu P Neupane and Williams Florence*
Department of Chemistry, College of Liberal Arts and Sciences, University of Iowa, Iowa City, IA
13. THERMOELECTRIC MITIGATION OF PSEUDOMONAS AERUGINOSA BIOFILMS
Parham Parnian and Eric Nuxoll*
Department of Chemical and Biochemical Engineering, College of Engineering, University of Iowa, Iowa City, IA
14. ZWITTERIONIC POLYMER COATINGS FOR DECREASED PROTEIN ADSORPTION
Christopher Ruben, Nolan Burson, Barrin Hendricks, and Jennifer Fiegel*
Department of Chemical and Biochemical Engineering, College of Engineering, University of Iowa, Iowa City, IA
15. SECRETED FACTORS FROM ADIPOCYTE LINEAGE CELLS DIFFERENTIALLY IMPACT FIBROBLAST TO MYOFIBROBLAST CONVERSION
Noah Sinclair1, Jesse Liszewski1, Mariam Y. El-Hattab1, Al Klingelhutz2, James A. Ankrum1, and Edward A. Sander1*
1Department of Biomedical Engineering, College of Engineering, University of Iowa, Iowa City, IA
2Department of Microbiology and Immunology. Carver College of Medicine, University of Iowa, Iowa City, IA
16. YEATS2 AND ZZZ3 ARE UPREGULATED IN SARCOMA CELL LINES SUGGESTING THE ATAC COMPLEX IS A KEY ONCOGENIC DRIVER
Souradip Sinha1,2, Keith Garcia1,3, Munir Tanas1,2,3*
1Department of Pathology, Carver College of Medicine, University of Iowa, Iowa City, IA
2Molecular Medicine Graduate Program, Carver College of Medicine, University of Iowa, Iowa City, IA
3Cancer Biology Graduate Program, University of Iowa Hospitals and Clinics, University of Iowa, Iowa City, IA
17. IMPROVING WOUND INFECTION TREATMENT USING SPRAYABLE, ANTIMICROBIAL HYDROGELS
Riannon Smith1, Nicole Brogden2 and Jennifer Fiegel1,2*
1Department of Chemical and Biochemical Engineering, College of Engineering, University of Iowa, Iowa City, IA
2Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, University of Iowa, Iowa City, IA
18. INVESTIGATING E-CADHERIN MEDIATED FORCE TRANSMISSION
Rebecca L. Splitt, Alicia Salvi, Hannah Campbell, and Kris DeMali*
Department of Biochemistry and Molecular Biology, Carver College of Medicine, University of Iowa, Iowa City, IA
19. development of a High-Throughput Screening method to investigate interactions between G-protein coupled receptors (GPCRs) and E. coli
Christopher Vidmar1, Joshua Wilkinson2, David Roman2, and Jennifer Fiegel1*
1Department of Chemical and Biochemical Engineering, College of Engineering, University of Iowa, Iowa City, IA.
2Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, University of Iowa, Iowa City, IA
20. INVESTIGATION OF MECHANISM OF NEUROTROPHIC HENYLBUTENOID DIMERS
Piyumi Wijesirigunawardana1, Khyati Gohil2 and Florence Williams1*
1Department of Chemistry, College of Liberal Arts and Sciences, University of Iowa, Iowa City, IA
2Department of Chemistry, University of Alberta, Edmonton, AB, Canada
21. THERMAL MITIGATION OF PSEUDOMONAS AERUGINOSA BIOFILMS GROWN IN VIVO
Konna Zoga1, Steven Bullard2, David A. Stoltz2, and Eric Nuxoll1*
1Department of Chemical and Biochemical Engineering, College of Engineering, University of Iowa, Iowa City, IA
2Department of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, IA
22. RAPID IDENTIFICATION and production of metabolites, AND ITS OPTIMIZATION USING HUMAN CYTOCHROME P450 2D6 (CYP2D6) ENGINEERED IN Thermothelomyces heterothallica (formerly Myceliopthora thermophila), (C1)
Shuvendu Das1, 2, Reid Hogan1, Ronen Tchelet3, Mark Emalfarb3, and Mark Arnold1, 2*
1Center for Biocatalysis and Bioprocessing, Office of Vice President for Research, University of Iowa Research Park, Coralville, IA
2Department of Chemistry, College of Liberal Arts and Sciences, University of Iowa, Iowa City, IA
3Dyadic International, Inc. 140 Intracoastal Pointe Drive, Suite # 404, Jupiter, Fl
23. SWSAP1-SWS1 functions to stimulate RAD51-mediated repair following alkylation damage
Sarah Hengel 1,2, Katherine Oppenheimer,1,2 and Kara Bernstein1,2
1Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, PA
2UPMC Hillman Cancer Center, Pittsburgh, PA, correspondence SAH202@pitt.edu
24. DETECTION OF G-QUADRUPLEX DNA STRUCTURES AT SINGLE-MOLECULE LEVEL USING G- QUADRUPLEX BINDING PROTEIN, G4P
Paras Gaur, Fletcher E Bain and Maria Spies*
Department of Biochemistry and Molecular Biology, Carver College of Medicine, University of Iowa, Iowa City, IA
Instructions for abstracts:
Please contact Mitchell Rotman if you have any questions. Abstracts may be submitted via email to Mitch Rotman (mitchell-rotman@uiowa.edu) .
Abstracts Due:
ABSTRACTS
(1) Use a 12 point font, Times or Times Roman and "exactly 12 pt" spacing of lines.
(2) The margins are: top 1.5", left 1.5", right 1" and bottom 1".
(3) The title of the paper should be bold faced and ALL CAPS (except where smaller case letters are appropriate to the meaning, example CaM).Leave a single blank line, then type the author's name(s) (first, middle initial,last). Please underline the name of the presenter/s (can you please indicate in your e-mail to me the following: if the presenter/s is a Undergraduate, Graduate, Research Scientist, etc.) and place an asterisk by the name of the faculty member or senior author (e.g., Rhonda Newman and Madeline Shea*). On the following line, type the departmental or company affiliation.
(4) Leave a single blank line and type the abstract using exactly spacing.
Here are a few sample abstracts from last year's poster award winners. PLEASE format your title, authors and affiliations using the format below. Other samples of abstracts can be found in the archived section at https://cbb.research.uiowa.edu/cbb-conference/archives
MAPPING THE METABOLIC PHENOTYPE OF TYPE II DIABETES MELLITUS IN HUMAN AQUEOUS HUMOR
Gabe Stanforth1,2, Jessica M. Skeie1,2,3, Christopher R. Fortenbach1,2, Darryl Y. Nishimura1,2,3, Gregory A. Schmidt2,3, Markus H. Kuehn1,4, and Mark A. Greiner1,2,3* 1Department of Ophthalmology and Visual Sciences, Carver College of Medicine, University of Iowa, Iowa City, IA 2Iowa Lions Eye Bank, BioVentures Center, Coralville, IA 3Cornea Research Center, University of Iowa, Iowa City, IA 4Center for the Prevention and reatment of Visual Loss, Iowa City Veterans Affairs Health Care System, Iowa City, IA
Our goal is to characterize the aqueous humor metabolome and proteome of humans with type II diabetes mellitus compared to controls, and identify differentially expressed proteins in specific diabetic disease states that may influence corneal endothelial cell (CEC) health.
Aqueous humor was biopsied from the anterior chamber of human eye donors (6 with advanced diabetes, 5 with nonadvanced diabetes, 5 nondiabetic controls) ≤5 hours postmortem using a 23-gauge needle or aqueous humor samples were collected from patients undergoing cornea transplant surgery. Protein fractions were isolated from each sample and subjected to multidimensional liquid chromatography and tandem mass spectrometry. Peptide spectra were analyzed statistically for largest differences associated with disease state and further bioinformatically for related mechanisms. We identified 1,003 differentially expressed protein isoforms including known risk factors for retinal diseases related to oxidative stress, inflammation, and the complement cascade (P<0.05). Gene ontology analysis showed diabetes disease progression has many protein footprints involved in binding, catalytic activity, and metabolic processes. Some of the most represented pathways involved in diabetes progression include acute phase response signaling, retinoid X receptor activation, complement system, and metabolism signaling.
This proteomic dataset gives insight into the mechanisms involved in diabetes disease progression relevant to adjacent structures including the corneal endothelium. These findings help prioritize new pathways for therapeutic targeting, and provide insight into potential biomarkers for determining anterior chamber health. Since diabetes is a metabolic disease and metabolism signaling was one of the most differentially expressed pathways in donors with varying degrees of diabetes, we are looking further into the aqueous humor changes using specific metabolomic assays and proteomic validation screens with human surgical aqueous samples.
NETWORKS OF COUPLED-RESIDUES PROMOTING CATALYSIS MAY BE A CHARACTERISTIC OF ALL ENZYMES
Nicholas A. Luedtke, Lauren K. Lambach and Christopher M. Cheatum* Department of Chemistry, College of Liberal Arts and Sciences, University of Iowa, Iowa City, IA
Enzymes have dynamic, internal motions on the timescales of femtoseconds to seconds which recruit residues across the protein. Femtosecond-picosecond motions can occur at the active site and may be linked to a coinciding event on the same timescale: catalysis. Protein motions are known to be linked to orchestrated motions of residues, called networks of coupled-residues. We hypothesize protein motions are linked to catalysis through networks of coupled-residues and this structure-function relationship is a classifying feature of all enzymes. We are using formate dehydrogenase and dihydrofolate reductase as model enzymes for predicting networks of coupled residues with the elastic network model and molecular dynamics simulations, two-dimensional infrared spectroscopy, steady-state kinetics, and crystallography to assess the validity of predicted networks in our model enzymes.
Dehalococcoides sp. reductive dehalogenase functional genes as potential biomarkers in anaerobic PCB-contaminated sediment microcosms
Jessica M. Ewald, Shelby V. Humes, Andres Martinez, Jerald L. Schnoor, and Timothy E. Mattes* Department of Civil and Environmental Engineering, IIHR-Hydroscience and Engineering, University of Iowa, Iowa City, IA
Polychlorinated biphenyls (PCBs) contaminate 19% of US superfund sites and represent a serious risk to human and environmental health. One promising strategy to remediate PCB contaminated sediments utilizes organohalide respiring bacteria (OHRB) that dechlorinate PCBs. However, functional genes that act as biomarkers for PCB dechlorination processes (i.e. reductive dehalogenase genes) are poorly understood. We developed anaerobic sediment microcosms that harbor an OHRB community dominated by the genus Dehalococcoides. During the 430-day microcosm incubation, Dehalococcoides 16S rRNA sequences increased two orders of magnitude to 107 copies/gram of sediment, and at the same time PCB118 decreased by as much as 70%. In addition, the OHRB community dechlorinated a range of penta- and tetra-chlorinated PCB congeners including PCBs 66, 70+74+76, 95, 90+101, and PCB110 without exogenous electron donor. We quantified candidate reductive dehalogenase (RDase) genes over a 430-day incubation period and found rd14, a reductive dehalogenase that belongs to Dehalococcoides mccartyi strain CG5, was enriched to 107 copies/gram of sediment. At the same time, pcbA5 was enriched to only 105 copies/gram of sediment. A survey for additional RDase genes revealed sequences similar to strain CG5’s rd4, and rd8. Ongoing work aims to quantify these rd14 transcripts in sediment free microcosms that have demonstrated PCB dechlorination after multiple transfers. In addition to demonstrating the PCB dechlorination potential of native microbial communities in contaminated freshwater sediments, our results suggest candidate functional genes with previously unexplored potential could serve as biomarkers of PCB dechlorination processes.