DEPT: Pharmaceutical Sciences and Experimental Therapeutics (PSET)
YEAR OF GRAD STUDY: 2009-2010
MENTOR(S): Dr. David L. Roman
SOURCE(S) OF SUPPORT: The Center for Biocatalysis and Bioprocessing (CBB) NIH Fellowship, 2011-2013.
The American Foundation for Pharmaceutical Education (AFPE) Pre-Doctoral Fellowship in Pharmaceutical Science, 2012-2014.
College of Pharmacy Dissertation Fellowship 2013-2014
The University of the Cumberlands B.S. 2006 Mathematics
The University of Iowa Ph.D. 2015 Medicinal & Natural Products Chemistry
ASPET _Graduate Student Travel Award to the ASPET Annual Meeting at Experimental Biology 2014, April 26th 2014
Professional Allocation Committee Travel Award to the ASPET Annual Meeting at Experimental Biology 2013,
Executive Council of Graduate & Professional Students, April 19-25th 2013
The Joseph G. Cannon Excellence in Graduate Research Travel Award, Division of Medicinal and Natural Products Chemistry, 2012-2013
ASPET Graduate Student Travel Award to the ASPET Annual Meeting at Experimental Biology 2011, April 9th 2011
Graduate Student Senate (GSS) Travel Funds Award to the ASPET annual meeting at Experimental Biology 2011, April 9th 2011
RESEARCH EXPERIENCE (prior to entering program):
CURRENT RESEARCH ACTIVITIES (brief description): Currently I am working on the investigation of a family of proteins called Receptor Activity Modifying Proteins or RAMPs. These proteins have been identified for their activity in regulating the transport and ligand specificity of the calcitonin-receptor-like receptor. While there have been a few G-Protein Coupled Receptors identified to interact with RAMPs, a thorough interrogation of the GPCR-ome has never been undertaken. My current project in Dr. Kathleen Caron’s laboratory is to develop a novel methodology to identify unique GPCR: RAMP interacting partners and to determine the investigate the results of these interactions. This work will expand our knowledge of GPCR signaling as well as identify novel protein: protein for possible pharmacological intervention.
POSTDOCTORAL TRAINING APPLIED FOR, UNDERWAY, OR COMPLETED:
University of Carolina- CH Dr. Kathleen Caron Cell biology and Physio. Nov. 2015 Nov. 2015
APPLICATION(S) FOR FUNDING (BUT THIS PROGRAM): None
PUBLICATIONS: PLEASE LIST BELOW AND CONTINUE ON THE BACK OF THIS FORM:
Mackie, D. I., Bodle, C.R., Hayes, M.P., Houtman, J.C.D., and Roman, D. L.: Discovery and characterization of the first cellularly active, non-covalent inhibitors of Regulator of G Protein Signaling 17. Molecular Cancer Therapeutics (MCT). 2014. (Submitted/Under review)
Zhang, X., Hagen, J., Muniz, V. P., Smith, T., Coombs, G. S., Eischen, C. M., Mackie, D. I., Roman, D. L., Van Rheeden, R., Darbro, B., Tompkins, V. S. and Quelle, D. E.: RABL6A, a novel RAB-like protein, controls centrosome amplification and chromosome instability in primary fibroblasts. PLoS One. 2013 Nov 25;8(11):e80228. doi: 10.1371/journal.pone.0080228. PMID: 24282525
*Bodle, C.R., *Mackie, D. I., and Roman, D. L.: Regulator of G Protein Signaling 17: An Emerging Therapeutic Target. Future Medicinal Chemistry. 2013. Jun; 5(9): 995-1007. doi: 10.4155/fmc.13.91. PMID: 23734683. (*Co-First Authorship)
Monroy, C.A., Mackie, D. I., and Roman, D. L.: A High Throughput Screen for RGS Proteins Using Steady State Monitoring of Free Phosphate Formation. PLoS One. 2013 Apr 23;8(4): e62247. doi: 10.1371/journal.pone.0062247. Print 2013. PMID: 23626793.
Mackie, D. I. and Roman, D. L.: Development of a Novel High-Throughput Screen and Identification of Small-Molecule Inhibitors of the G-RGS17 Protein-Protein Interaction Using AlphaScreen. J Biomol Screen. 2011 Sep; 16(8): 869-77. doi: 10.1177/1087057111410427. Epub 2011 Jun 16. PMID: 21680864.