The Center for Biocatalysis and Bioprocessing has the capacity to offer a wide range of professional and research-based services such as gene cloning, clonal selection, fermentation, downstream purification, bioprocessing, and other various instrumental methods to serve local, national, and international clientele. Request Service Now!Follow Us on LinkedIn to Get Notifications from Us and Learn More about Our Services!
We offer an experienced professional staff and state-of-the-art fermentation and protein purification facilities to perform confidential contract services. Operations are smoothly transferred from the main facility to GMP. In 2004, CBB acquired the current Good Manufacturing Practices (cGMP) facility with three protein purification suites. In 2007, CBB completed a cGMP fermentation suite containing 30, 100 and 300-liter fermentors. The GMP facilities are located on the University of Iowa Research Park adjacent to the CBB's Research and Process Development (RPD) Laboratory.
For upstream processing in our RPD Laboratory, the facility contains various shakers such as Innova multi-tier shakers and lab companion shakers for seed growth and media optimization. Fermentation scale-up optimization can be performed within a family of microbial fermentors with working volumes of 5, 30, 100, and 1000 L. The 5 L fermentors belong to a BioStat B array of 4 × 5 glass fermentors. In total, the RPD lab includes four 30 L, two 100 L and one 1000 L stainless steel fermentors. All these fermentors are fully equipped with modern functions and features, such as built-in peristaltic pumps and digital measurement and control systems, including probes for temperature, pH, dissolved oxygen and foam. Harvest options can be evaluated using various high-speed, high-capacity centrifuges that offer precise speed control and high reproducibility. Examples include a AS-26 Sharples, a Disc Stack Alfa-Laval LAPX 404, and a Sorvall LYNX 6000 floor centrifuge.
For downstream processing in our RPD Laboratory, numerous downstream options are available for product recovery at various scales. Intracellular products can be released by using either a M-110Y or M-210-EH Microfluidizer depending on volume of the fermentation product. A series of ÄKTA chromatography systems is available to facilitate protein purification. The ÄKTA Purifier 100, ÄKTA Explorer 100, ÄKTA Pilot 600, ÄKTA Avant 150, and ÄKTA Process are available for different scales of preparative column chromatography. The CBB maintains a limited number of different size chromatography columns for executing client projects. Multiple Tangential Flow Filtration (TFF) systems of various scale are available to perform buffer exchange, solute concentration, ultrafiltration or microfiltration with volumes up to 100’s of liters. Various spectrometers are available for analysis of in-process samples. Units include a Shimadzu UV-2401, Mettler Toledo UV5Nano, and Bio-Rad SmartSpec Plus spectrometers. A Bio-Rad GS900 densitometer is also available to image SDS-PAGE gels and estimate protein yield across a wide dynamic range. An Endosafe Nexgen-PTS is used for routine monitoring of water systems and materials to help minimize endotoxins levels in final products.
Equipment in the cGMP laboratory is nearly identical to selected equipment located in the non-GMP laboratory. This design is intended to permit a seamless transfer of a project from the non-GMP lab and into the cGMP lab. The cGMP lab contains two stainless steel fermentors, with working volumes of 30 and 100 L. Cell paste is harvested with either an Alfa-Laval or Sorvall Evolution RC centrifuge unit. For downstream operations, cells are ruptured with an M-110Y microfluidizers, depending on fermentation volume. Preparative chromatography is performed by using an ÄKTA Pilot system coupled with appropriate volume columns. When needed, multiple Tangential Flow Filtration (TFF) systems are available for ultrafiltration, microfiltration, buffer exchange, and solute concentration. This cGMP space is monitored continuously for temperature, pressure, and particle counts. In addition, this space has its own, isolated HVAC system as well as dedicated compressor units and clean stream supply.
CBB is committed to working collaboratively with clients from all over the world to deliver their products. CBB interacts with more than 60 different clients, from start-up to big pharma to the nation's foremost universities and governmental agencies, each year. An experienced expert staff operates CBB’s state-of-the-art upstream and downstream processing facilities. The CBB develops, evaluates, optimizes, and scales up bio-based products in volumes ranging from 1 to 1,000-L. Typically, they bring research findings from the bench through the first stages of scale-up. The more than 20-member lab staff's extensive experience includes a wide-range of organisms, notably high-cell-density recombinant E.coli and Pichia pastoris. Products produced cover the complete range of interest - vaccines, enzymes, immobilized catalysts, antibiotics, therapeutics, nucleotides, polysaccharides, peptides, polymers, and biochemicals. The development process is rapid, economical and can be easily transferred to CBB cGMP Laboratory. CBB produces bulk biopharmaceuticals for evaluation, with considerable experience purifying fermentation-produced biological materials. Typical operations may include diafiltration, ultrafiltration, and complex chromatographic separations.
Clients find the CBB's business practices fast, efficient and easy. Typical projects start with a Confidentiality Agreement, allowing the exchange of necessary information. CBB follows this with a Service Agreement and/or proposal outlining the protocol, estimated costs and timing. To request a service, please fill out this Contract Service Request Form.