Baculovirus/Insect Cell Expression Service

CBB also offers expression service with the Baculovirus/Insect Cell Expression Vector System for complex proteins. Baculovirus/Insect Cell expression system is a well-proven expression system for proteins that need glycosylation, and proper folding. Advantages of this expression system include:

  • Good expression level
  • Capablility to express proteins both internally or by secretion
  • Can perform eukaryotic post-translational modification
  • Insect cells can be adapted to grow in suspension, easy to scale-up
  • Faster production time
  • Safe from human viruses

CBB expression service with Baculovirus/Insect Cells includes:

  • Subcloning of the gene of interest into commercially available expression vector
  • Transfect insect cell culture with the expression construct
  • Recovery of recombinant virus from primary infection
  • Amplification of recombination virus, plaque purification, and viral titer determination
  • Optimization of protein expression in small-scale shake flask study
  • Scale-up from 1-100-L and downstream processing

Please contact us for question or inquiry about Baculovirus/Insect Cell Expression service at CBB.